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KMID : 0379520050210040291
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한국독성학회지
2005 Volume.21 No. 4 p.291 ~ p.295
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Silica Induced Phospholipase D (PLD) Activation in Rat2 Fibroblasts
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Ahn Eun-Kyung
Lim Oh-Kyung
Nam Hae-Yun
Kim Hyung-Jung
Chung Nam-Hyun
Bae Gwi-Nam
Lim Young
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Abstract
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To define the effect of silica on the stimulator of signaling pathway, we studied the phospholipase D (PLD) activity in the Rat2 fibroblasts. Silica stimulated the accumulation of labeled [^3H] phosphatidylethanol([^3H];PEt) in a time- and concentration-dependent manner. This Silicainduced PLD activity was partially attenuated by the pretreatment with U73122 (phospholipase C inhibitor), genistein (protein tyrosine kinase inhibitor), PD 98056 (MEK inhibitor) and mepacrine (phospholipase A_2 inhibitor). But, sphingosine (protein kinase C inhibitor) and DPI (NADPH reductase inhibitor) had not effect the PLD activity. Silica also increased the PLD activity about four fold, which imply that the PLD activity is more influenced by the mobilization of PLD than other signaling mediators. The PLD activity also partially inhibited calcium chelator EGTA or/and BAPTA/AM compared to silica. Finally, we concluded that a silica-stimulated phospholipase D activity is present in the Rat2 fibroblasts and is modulated by combination of various signaling mediators.
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KEYWORD
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PLD, Silica, Rat2 fibroblast, Signaling mediator, Silicosis
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